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Contents: Electrophoresis 22'12
Author(s) -
Lilla Makszin,
Anikó Kilár,
Péter Felső,
Zoltán Péterfi,
Béla Kocsis,
Ferenc Kilár
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201270163
Subject(s) - electrophoresis , chromatography , staining , silver stain , chemistry , bacteria , covalent bond , fluorescence , microbiology and biotechnology , biology , genetics , physics , organic chemistry , quantum mechanics
A novel, fast, and sensitive ME method was developed to analyze and differentiate the smooth (S) and rough (R) type bacterial endotoxin components labeled covalently with a fluorescent dye. The quantitative analysis of purified lipopolysaccharides, or partially purified samples from whole-cell lysates becomes possible with this method. Two groups with three sub-groups in the first group of S-type lipopolysaccharides can be classified based on the electrophoretic profiles. The LOD of the endotoxins from S- and R-type Gram-negative bacteria was found to be 2.6 ng and 6.9 ng, respectively. This method is capable to replace the commonly used SDS-PAGE combined with silver staining.