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Determination of carbohydrate‐deficient transferrin in human serum by capillary zone electrophoresis: Evaluation of assay performance and quality assurance over a 10‐year period in the routine arena
Author(s) -
Joneli Jeannine,
Wanzenried Ursula,
Schiess Jeannette,
Lanz Christian,
Caslavska Jitka,
Thormann Wolfgang
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200653
Subject(s) - chromatography , transferrin , capillary electrophoresis , carbohydrate deficient transferrin , quality assurance , chemistry , detection limit , transferrin saturation , external quality assessment , medicine , biochemistry , pathology , alcohol , alcohol consumption , serum ferritin , ferritin
The performance of high‐resolution CZE for determination of carbohydrate‐deficient transferrin ( CDT ) in human serum based on internal and external quality data gathered over a 10‐year period is reported. The assay comprises mixing of serum with a Fe(III) ion‐containing solution prior to analysis of the iron saturated mixture in a dynamically double‐coated capillary using a commercial buffer at alkaline pH. CDT values obtained with a human serum of a healthy individual and commercial quality control sera are shown to vary less than 10%. Values of a control from a specific lot were found to slowly decrease as function of time (less than 10% per year). Furthermore, due to unknown reasons, gradual changes in the monitored pattern around pentasialo‐transferrin were detected, which limit the use of commercial control sera of the same lot to less than 2 years. Analysis of external quality control sera revealed correct classification of the samples over the entire 10‐year period. Data obtained compare well with those of HPLC and CZE assays of other laboratories. The data gathered over a 10‐year period demonstrate the robustness of the high‐resolution CZE assay. This is the first account of a CZE ‐based CDT assay with complete internal and external quality assessment over an extended time period.