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Proteomic analysis of Lycium barbarum (Goji) fruit via combinatorial peptide ligand libraries
Author(s) -
D'Amato Alfonsina,
Esteve Clara,
Fasoli Elisa,
Citterio Attilio,
Righetti Pier Giorgio
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200643
Subject(s) - lycium , proteome , chemistry , acetone , solubilization , chromatography , ligand (biochemistry) , peptide , pulmonary surfactant , biology , food science , botany , biochemistry , receptor , medicine , alternative medicine , pathology
The proteome of Goji berries ( Lycium barbarum ) has been investigated in depth by using the combinatorial peptide ligand libraries (CPLL) technology. Since, in general, when plant samples are solubilized under native conditions, a limited number of identifications ensues, we have tried here solubilization under both native and denaturing conditions. In the latter case, since up to 3% SDS is used, the amount of surfactant is diluted to 0.1% or else proteins are precipitated with acetone/methanol, followed by CPLL capture. Under these conditions, a total amount of 350 proteins has been univocally identified, which represents by far the deepest proteomic investigation of Goji berries.