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Targeted analysis of omega‐6‐derived eicosanoids in human serum by SPE‐LC‐MS/MS for evaluation of coronary artery disease
Author(s) -
Fernández Peralbo María Auxiliadora,
PriegoCapote Feliciano,
GalacheOsuna José Gabriel,
Luque de Castro María Dolores
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200603
Subject(s) - coronary artery disease , myocardial infarction , medicine , aspirin , acute coronary syndrome , thromboxane a2 , cardiology , thromboxane b2 , unstable angina , thromboxane , gastroenterology , platelet
A targeted approach has been applied to quantitative analysis of eicosanoids derived from omega‐6 fatty acids in serum from individuals diagnosed with coronary artery disease (CAD). The target metabolites were series‐2 prostaglandins, thromboxane B2, hydroxy‐eicosatetraenoic acids, and hydroxyoctadecadienoic acids. The method was based on SPE‐LC‐MS/MS in selected reaction monitoring mode for highly selective and sensitive determination of the target eicosanoids. The combination of SPE and LC‐MS/MS involved the benefits from both direct analysis of serum without a step for protein precipitation and fully automation of the analysis. The method allowed comparison of omega‐6‐derived eicosanoids in serum from patients diagnosed with CAD and from control individuals. The effect of treatment with aspirin on the profile of the target compounds was evaluated through its incidence on the different pathways. Finally, the serum levels of the target metabolites in patients diagnosed with CAD were also statistically examined according to the severity of the coronary lesion stratified as stable angina, non‐ST‐elevation acute coronary syndrome, and acute myocardial infarction.