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Live cell refractometry based on non‐ SPR microparticle sensor
Author(s) -
Liu Chang,
Chen David D. Y.,
Yu Lirong,
Luo Yong
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200593
Subject(s) - differential interference contrast microscopy , refractometry , refractive index , cytoplasm , microscopy , surface plasmon resonance , interference microscopy , materials science , wavelength , interference (communication) , optics , microparticle , bright field microscopy , optical microscope , chemistry , nanoparticle , biophysics , optoelectronics , nanotechnology , scanning electron microscope , physics , biology , biochemistry , channel (broadcasting) , electrical engineering , engineering
Unlike the nanoparticles with surface plasmon resonance, the optical response of polystyrene microparticles ( PSMP s) is insensitive to the chemical components of the surrounding medium under the wavelength‐dependent differential interference contrast microscopy. This fact is exploited for the measurement of the refractive index of cytoplasm in this study. PSMP s of 400 nm in diameter were loaded into the cell to contact cytoplasm seamlessly, and the refractive index information of cytoplasm could be extracted by differential interference contrast microscopy operated at 420 nm illumination wavelength through the contrast analysis of PSMP s images.