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Micellar ordered structure effects on high‐resolution CE‐SSCP using P luronic triblock copolymer blends
Author(s) -
Hwang Hee Sung,
Shin Gi Won,
Park Han Jin,
Ryu Chang Yeol,
Jung Gyoo Yeol
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200520
Subject(s) - poloxamer , copolymer , micelle , ethylene oxide , amphiphile , materials science , chemical engineering , polymer , propylene oxide , aqueous solution , polymer chemistry , chemistry , organic chemistry , composite material , engineering
P luronic F108 block copolymers have shown a great promise to achieve the desirable high resolution in the conformation‐sensitive separation of ss DNA using CE‐SSCP . However, fundamental understanding of the structures and properties of P luronic matrix affecting the resolution is still limited. Unlike conventional gel‐forming homopolymers, P luronic F108 block copolymers are amphiphilic macromolecules consisting of poly(ethylene oxide)‐ b ‐poly(propylene oxide)‐ b ‐poly(ethylene oxide) triblock copolymers, which are capable of forming a highly ordered micellar structure in aqueous solution. In this study, we have performed a series of experiments by blending different types of P luronic polymers to control the formation of micelles and to study the correlation between separation and rheological characteristics of P luronic gels affecting the resolution of CE‐SSCP . Our experiments have been specifically designed to elucidate how the micellar structure affects the resolution of CE‐SSCP upon altering the size uniformity and constituent homogeneity of the micelles. Our results suggest that uniformly sized micelle packing is the primary structural feature of P luronic gel matrix for the high‐resolution separation, while the size and constituent of the micelle themselves need to be considered as secondary factors.