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Label‐free aptamer‐based partial filling technique for enantioseparation and determination of dl ‐tryptophan with micellar electrokinetic chromatography
Author(s) -
Huang Rong,
Xiong Wenming,
Wang Daifang,
Guo Longhua,
Lin Zhenyu,
Yu Lishuang,
Chu Kedan,
Qiu Bin,
Chen Guonan
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200464
Subject(s) - aptamer , micellar electrokinetic chromatography , chromatography , enantiomer , chemistry , electrokinetic phenomena , sodium cholate , tryptophan , micelle , high performance liquid chromatography , capillary electrophoresis , aqueous solution , organic chemistry , amino acid , biochemistry , genetics , biology
In this study, a simple and reproducible method for enantioseparation and determination of dl ‐tryptophan ( dl ‐ T rp) was developed by using a partial filling technique in combination with MEKC . The corresponding l ‐ T rp specific DNA aptamer was used as a chiral selector. Sodium cholate was used to form the chiral micelles and to enhance the enantioseparation of the enantiomers. Effects of aptamer concentration, filling time, buffer composition, and separation voltage on the enantioseparation were evaluated. The M g 2+ and Na + concentration in separation buffer was found to effectively affect the separation efficiency and reproducibility. Under the optimal conditions, d ‐ and l ‐ T rp were completely enantioseparated in less than 9 min. This aptamer‐based partial‐filling approach has the potential to be extended to the separation of other enantiomers after the replacement of corresponding specific aptamers.