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Development and optimization of an integrated PDMS based‐microdialysis microchip electrophoresis device with on‐chip derivatization for continuous monitoring of primary amines
Author(s) -
Nandi Pradyot,
Scott David E.,
Desai Dhara,
Lunte Susan M.
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200454
Subject(s) - derivatization , chromatography , analyte , microdialysis , electrophoresis , chemistry , amine gas treating , capillary electrophoresis , resolution (logic) , analytical chemistry (journal) , high performance liquid chromatography , organic chemistry , biochemistry , extracellular , computer science , artificial intelligence
An all‐ PDMS on‐line microdialysis‐microchip electrophoresis with on‐chip derivatization and electrophoretic separation for near real‐time monitoring of primary amine‐containing analytes is described. Each part of the chip was optimized separately, and the effect of each of the components on temporal resolution, lag time, and separation efficiency of the device was determined. Aspartate and glutamate were employed as test analytes. Derivatization was accomplished with naphthalene‐2,3,‐dicarboxyaldehyde/cyanide ( NDA / CN − ), and the separation was performed using a 15‐cm serpentine channel. The analytes were detected using LIF detection.