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Improving the analysis of Y ‐ SNP haplogroups by a single highly informative 16 SNP multiplex PCR ‐minisequencing assay
Author(s) -
Valverde Laura,
Köhnemann Stephan,
Cardoso Sergio,
Pfeiffer Heidi,
de Pancorbo Marian M.
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200433
Subject(s) - haplogroup , snp , subclade , biology , genetics , multiplex , single nucleotide polymorphism , y chromosome , multiplex polymerase chain reaction , human mitochondrial dna haplogroup , snp array , mitochondrial dna , haplotype , evolutionary biology , genotype , computational biology , polymerase chain reaction , gene , phylogenetic tree , clade
The SNP haplogroups of the Y ‐chromosome are nonrandomly distributed among human populations. They are used for tracing the phylogeographical history of paternal lineages of male individuals and can be a useful tool for approaching the patrilineal bio‐geographic ancestry of unknown forensic evidences. With the aim of facilitating the inference of the principal informative worldwide Y ‐ SNP haplogroups, we have selected the minimum possible number of key Y ‐ SNP s to be amplified in a sensitive single multiplex PCR and detected by minisequencing. This assay, that includes 16 Y ‐ SNP s, was tested for male human specificity, sensitivity, and reproducibility. Its effectiveness was assessed in a set of degraded DNA samples and in a panel of male individuals from different worldwide populations. All these tests demonstrated the convenience of this assay for assigning the major Y haplogroups to forensic evidences by one single PCR ‐minisequencing reaction.