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Genetic polymorphisms in 12 autosomal STR s in a S hanghai H an population from China
Author(s) -
Zhang Suhua,
Zhang Zheren,
Zhao Shumin,
Zhao Zhenmin,
Li Chengtao
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200423
Subject(s) - locus (genetics) , genetics , allele , biology , population , confidence interval , china , chinese population , microsatellite , polymorphism (computer science) , gene , demography , genotype , medicine , geography , sociology , archaeology
In this study, a total of 484 unrelated healthy individuals and 359 two‐generation families in the H an population in S hanghai, C hina were successfully analyzed with the I nvestigator HD plex K it (Qiagen, Hilden, Germany). H ardy– W einberg equilibrium tests demonstrated no significant deviation from expected values ( p > 0.05) for the 12 autosomal STR s included in this kit. A total of 173 alleles were detected in 12 autosomal STR loci; the largest number of alleles (32) was detected for the SE 33 locus. The combined power of discrimination was 0.2. The combined mean exclusion chance in duo cases was 0.99982, whereas the combined mean exclusion chance in trio cases was 0.86. The average mutation rate across all loci was 0.0023 (95% confidence intervals: 0.0013–0.0039). These results suggest that the 12 autosomal STR loci can provide highly informative polymorphic data for paternity testing and forensic identification in the H an population in S hanghai, C hina.