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An accurate multiplex antibiotic susceptibility test using a high‐resolution CE ‐ SSCP ‐based stuffer‐free multiplex ligation‐dependent probe amplification system
Author(s) -
Chung Boram,
Shin Gi Won,
Choi Woong,
Hwang Hee Sung,
Oh MiHwa,
Jung Gyoo Yeol
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200372
Subject(s) - multiplex ligation dependent probe amplification , multiplex , antibiotics , multiplex polymerase chain reaction , antimicrobial , biology , microbiology and biotechnology , polymerase chain reaction , genetics , gene , exon
The success of antimicrobial therapy depends on effective prescription of antibiotics. Assessment of clinical isolates using rapid antimicrobial susceptibility tests allows effective microbiological therapy to be commenced in a timely manner. However, conventional antimicrobial susceptibility testing is time‐consuming and laborious. In the present study, we employed stuffer‐free multiplex ligation‐dependent probe amplification ( MLPA ) coupled with analysis of single‐strand conformation polymorphisms, via high‐resolution CE , to develop a multiplex antibiotic susceptibility test. Using this method, parallel analysis of specific genetic markers was employed to determine minimal inhibitory concentration values. The values derived using the stuffer‐free MLPA method agreed with those estimated using a conventional broth dilution method. These findings indicate that the stuffer‐free MLPA ‐based approach is a viable alternative to the conventional method.