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Use of proteomic methods in the analysis of human body fluids in A lzheimer research
Author(s) -
Zürbig Petra,
Jahn Holger
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200360
Subject(s) - proteomics , proteome , computational biology , biological fluids , disease , organism , population , biology , bioinformatics , computer science , chemistry , medicine , pathology , biochemistry , genetics , chromatography , environmental health , gene
Proteomics is the study of the entire population of proteins and peptides in an organism or a part of it, such as a cell, tissue, or fluids like cerebrospinal fluid, plasma, serum, urine, or saliva. It is widely assumed that changes in the composition of the proteome may reflect disease states and provide clues to its origin, eventually leading to targets for new treatments. The ability to perform large‐scale proteomic studies now is based jointly on recent advances in our analytical methods. Separation techniques like CE and 2 DE have developed and matured. Detection methods like MS have also improved greatly in the last 5 years. These developments have also driven the fields of bioinformatics, needed to deal with the increased data production and systems biology. All these developing methods offer specific advantages but also come with certain limitations. This review describes the different proteomic methods used in the field, their limitations, and their possible pitfalls. Based on a literature search in P ub M ed, we identified 112 studies that applied proteomic techniques to identify biomarkers for A lzheimer disease. This review describes the results of these studies on proteome changes in human body fluids of A lzheimer patients reviewing the most important studies. We extracted a list of 366 proteins and peptides that were identified by these studies as potential targets in A lzheimer research.