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Recent advances in preparation and application of hybrid organic‐silica monolithic capillary columns
Author(s) -
Ou Junjie,
Lin Hui,
Zhang Zhenbin,
Huang Guang,
Dong Jing,
Zou Hanfa
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200344
Subject(s) - capillary action , materials science , capillary electrochromatography , chromatography , nanotechnology , chemistry , composite material
Hybrid organic‐silica monolithic columns, regarded as a second generation of silica‐based monoliths, have received much interest due to their unique properties over the pure silica‐based monoliths. This review mainly focuses on development in the fields of preparation of hybrid monolithic columns in a capillary and their application for CEC and capillary liquid chromatography separation, as well as for sample pretreatment of solid‐phase microextraction and immobilized enzyme reactor since J uly 2010. The preparation approaches are comprehensively summarized with three routes: (i) general sol–gel process using trialkoxysilanes and tetraalkoxysilanes as coprecursors; (ii) “one‐pot” process of alkoxysilanes and organic monomers concomitantly proceeding sol–gel chemistry and free radical polymerization; and (iii) other polymerization approaches of organic monomers containing silanes. The modification of hybrid monoliths containing reactive groups to acquire the desired surface functionality is also described.