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Effect of PVP on the electroosmotic mobility of wet‐etched glass microchannels
Author(s) -
Milanova Denitsa,
Chambers Robert D.,
Bahga Supreet S.,
Santiago Juan G.
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200336
Subject(s) - ionic strength , soda lime glass , electrophoresis , electro osmosis , capillary electrophoresis , polymer , analytical chemistry (journal) , coating , materials science , adsorption , chemistry , chemical engineering , chromatography , composite material , aqueous solution , organic chemistry , engineering
We present an experimental study on the effect of polymer PVP on EOF mobility of microchannels wet etched into optical white soda lime glass, also known as C rown glass. We performed experiments to evaluate the effect of PVP concentration and p H on EOF mobility. We used on‐chip capillary zone electrophoresis and a neutral fluorescent dye as a passive marker to quantify the electroosmotic flow. We performed experiments under controlled conditions by varying p H from 5.2 and 10.3 and concentration of PVP from 0 to 2.0% w/w at constant ionic strength (30 mM). Our experiments show that PVP at concentrations of 1.0% or above very effectively suppress EOF at low pH (6.6). At high pH of 10.3, PVP has a much weaker suppressing effect on EOF and increasing its concentration above about 0.5% showed negligible effect on EOF mobility. Finally, we briefly discuss the effects of p H on using PVP as an adsorbed coating. Our experiments provide useful guidelines on choosing correct p H and concentration of PVP for effective EOF suppression in glass channels.