2 D  difference gel electrophoresis reference map of a   F usarium graminearum  nivalenol producing strain | Zendy

Pasquali Matias | Zendy; Serchi Tommaso | Zendy; Renaut Jenny | Zendy; Hoffmann Lucien | Zendy; Bohn Torsten | Zendy

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2 D difference gel electrophoresis reference map of a F usarium graminearum nivalenol producing strain

Author(s) -

Pasquali Matias,

Serchi Tommaso,

Renaut Jenny,

Hoffmann Lucien,

Bohn Torsten

Publication year - 2013

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.201200256

Subject(s) - fusarium , chemotype , strain (injury) , biology , mycotoxin , microbiology and biotechnology , computational biology , chemistry , botany , anatomy , essential oil

Fusarium graminearum is widely studied as a model for toxin production among plant pathogenic fungi. A 2 D DIGE reference map for the nivalenol‐producing strain 453 was established. Based on a whole protein extract, all reproducible spots were systematically picked and analyzed by MALDI‐TOF/TOF , leading to the identification of 1102 protein species. The obtained map contributes to the annotation of the genome by identifying previously nondescribed hypothetical proteins and will serve as a reference for future studies aiming at deciphering F . graminearum biology and chemotype diversity.

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