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2 D difference gel electrophoresis reference map of a F usarium graminearum nivalenol producing strain
Author(s) -
Pasquali Matias,
Serchi Tommaso,
Renaut Jenny,
Hoffmann Lucien,
Bohn Torsten
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200256
Subject(s) - fusarium , chemotype , strain (injury) , biology , mycotoxin , microbiology and biotechnology , computational biology , chemistry , botany , anatomy , essential oil
Fusarium graminearum is widely studied as a model for toxin production among plant pathogenic fungi. A 2 D DIGE reference map for the nivalenol‐producing strain 453 was established. Based on a whole protein extract, all reproducible spots were systematically picked and analyzed by MALDI‐TOF/TOF , leading to the identification of 1102 protein species. The obtained map contributes to the annotation of the genome by identifying previously nondescribed hypothetical proteins and will serve as a reference for future studies aiming at deciphering F . graminearum biology and chemotype diversity.