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Electrophoretic separations in poly(dimethylsiloxane) microchips using mixtures of ionic, nonionic and zwitterionic surfactants
Author(s) -
Guan Qian,
Noblitt Scott D.,
Henry Charles S.
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200255
Subject(s) - pulmonary surfactant , nonionic surfactant , chemistry , chromatography , resolution (logic) , ionic bonding , electrophoresis , triton x 100 , analyte , analytical chemistry (journal) , organic chemistry , ion , biochemistry , artificial intelligence , computer science
The use of surfactant mixtures to affect both EOF and separation selectivity in electrophoresis with PDMS substrates is reported, and capacitively coupled contactless conductivity detection is introduced for EOF measurement on PDMS microchips. First, the EOF was measured for two nonionic surfactants (Tween 20 and Triton X‐100), mixed ionic/nonionic surfactant systems ( SDS /Tween 20 and SDS /Triton X‐100), and finally for the first time, mixed zwitterionic/nonionic surfactant systems ( TDAPS /Tween 20 and TDAPS /Triton X‐100). EOF for the nonionic surfactants decreased with increasing surfactant concentration. The addition of SDS or TDAPS to a nonionic surfactant increased EOF . After establishing the EOF behavior, the separation of model catecholamines was explored to show the impact on separations. Similar analyte resolution with greater peak heights was achieved with mixed surfactant systems containing Tween 20 and TDAPS relative to the single surfactant system. Finally, the detection of catecholamine release from PC 12 cells by stimulation with 80 mM K + was performed to demonstrate the usefulness of mixed surfactant systems to provide resolution of biological compounds in complex samples.