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Identification of avocado ( P ersea americana) pulp proteins by nano‐ LC ‐ MS / MS via combinatorial peptide ligand libraries
Author(s) -
Esteve Clara,
D'Amato Alfonsina,
Marina María Luisa,
García María Concepción,
Righetti Pier Giorgio
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200254
Subject(s) - persea , proteome , peptide , allergen , chemistry , pectinase , biology , biochemistry , botany , enzyme , allergy , immunology
Avocado ( P ersea americana ) proteins have been scarcely studied despite their importance, especially in food related allergies. The proteome of avocado pulp was explored in depth by extracting proteins with capture by combinatorial peptide ligand libraries at p H 7.4 and under conditions mimicking reverse‐phase capture at p H 2.2. The total number of unique gene products identified amounts to 1012 proteins, of which 174 are in common with the control, untreated sample, 190 are present only in the control and 648 represent the new species detected via combinatorial peptide ligand libraries of all combined eluates and likely represent low‐abundance proteins. Among the 1012 proteins, it was possible to identify the already known avocado allergen P ers a 1 and different proteins susceptible to be allergens such as a profilin, a polygalacturonase, a thaumatin‐like protein, a glucanase, and an isoflavone reductase like protein.