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Analysis of short‐chain aliphatic amines in food and water samples using a near infrared cyanine 1‐(ε‐succinimydyl‐hexanoate)‐1′‐methyl‐3,3,3′,3′‐tetramethyl‐indocarbocyanine‐5,5′‐disulfonate potassium with CE – LIF detection
Author(s) -
Fu NiNa,
Zhang HuaShan,
Wang Hong
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200237
Subject(s) - chemistry , chromatography , cyanine , derivatization , potassium , triarylmethane dye , phosphoric acid , calibration curve , phenol , detection limit , high performance liquid chromatography , fluorescence , organic chemistry , physics , quantum mechanics
Precolumn derivatization of six short‐chain aliphatic amines by a near‐infrared dye, 1‐(ε‐succinimydyl‐hexanoate)‐1′‐methyl‐3,3,3′,3′‐tetramethyl‐indocarbocyanine‐5,5′‐ disulfonate potassium (MeCy5‐OSu), followed by MEKC – CE – LIF detection has been developed as a method for the determination of aliphatic amines in environmental water and food. Optimum derivatization was operated nicely in pH 9.0 borate buffer at 20°C for 30 min. Well separated peaks were observed with a pH 9.5 BGE containing 10 mmol L −1 phosphoric acid, 20 mmol L −1 SDS , and 7% methanol buffered with 1.0 mol L −1 N a OH . The separation procedure was rapidly achieved within 11 min and the matrix interferences could be effectively eliminated. A linear calibration graph was obtained for 5–200 nmol L −1 analytes with a correlation coefficient in the range 0.9933–0.9995 for amines. This method was successfully utilized to determine aliphatic amines in lake, sewage water, and red wine with recoveries ranging from 96.4 to 105% and the RSD s ranging from 0.9 to 2.9%. Near‐infrared, LIF ‐detector‐compatible MeCy5‐OSu was proved suitable for the accurate, sensitive, and rapid separation and determination of aliphatic amines in water and food samples.