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Design and fabrication of a COP ‐based microfluidic chip: Chronoamperometric detection of T roponin T
Author(s) -
Abad Llibertat,
Javier del Campo Francisco,
Muñoz Francesc Xavier,
Fernández Luis J.,
Calavia Daniel,
Colom Gloria,
Salvador Juan P.,
Marco María Pilar,
EscamillaGómez Vanessa,
EstebanFernández de Ávila Berta,
Campuzano Susana,
Pedrero María,
Pingarrón José M.,
Godino Neus,
Gorkin Robert,
Ducrée Jens
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200225
Subject(s) - detection limit , microfluidics , fabrication , microfluidic chip , chromatography , materials science , chip , nanotechnology , electrode , chemistry , computer science , alternative medicine , medicine , telecommunications , pathology
This work demonstrates the design and fabrication of an all cyclo‐olefin polymer based microfluidic device capable of capturing magnetic beads and performing electrochemical detection in a series of gold electrodes. The size of chip is of a microscope slide and features six independent measuring cells for multianalyte detection purposes. The aim of this work is to show that rapid prototyping techniques can be instrumental in the development of novel bioassays, particularly in clinical diagnosis applications. We show the successful determination of troponin‐ T , a cardiac disease marker, in the clinically relevant range of 0.05–1.0 ng/mL. This methodology achieves a detection limit of 0.017 ng/mL in PBS solutions, and is capable of detecting less than 1 ng/mL in a 1:50 human serum dilution.