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IEF‐based multidimensional applications in proteomics: Toward higher resolution
Author(s) -
Stoyanov Alexander
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200221
Subject(s) - resolution (logic) , computer science , proteomics , chromatography , characterization (materials science) , chemistry , artificial intelligence , nanotechnology , materials science , biochemistry , gene
This review focuses on the alternative isoelectrofocusing methods and IEF ‐related techniques in protein analysis and characterization. The main emphasis is placed on the evaluation of the resolving power of new approaches, as well as potential advantages of IEF ‐related techniques for multidimensional analysis. In particular, the inverse 2 D gel electrophoresis where the M w separation stage precedes IEF mode is considered.