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Developing an amperometric hydrogen peroxide sensor for an exhaled breath analysis system
Author(s) -
Wiedemair Justyna,
van Dorp Henriëtte D. S.,
Olthuis Wouter,
van den Berg Albert
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200218
Subject(s) - amperometry , detection limit , electrode , analytical chemistry (journal) , hydrogen peroxide , working electrode , electrochemical gas sensor , cyclic voltammetry , electrolyte , materials science , flow injection analysis , microfabrication , electrochemistry , chemistry , chromatography , fabrication , organic chemistry , medicine , alternative medicine , pathology
In this work, we present a chip‐integrated amperometric sensor targeted at the detection of hydrogen peroxide (H 2 O 2 ) in the gaseous phase. Electrode chips are manufactured in a series of microfabrication steps and characterized electrochemically. Using such devices detection of H 2 O 2 in an aqueous phase is shown by means of cyclic voltammetry and amperometry. Furthermore, it is discussed that variation of conditions such as the composition of the supporting electrolyte largely influences the obtained electrochemical signal. Additionally, electrochemical pretreatment of platinum working electrodes aiming at surface oxidation improves the limit of detection of the sensor and the linearity of the calibration curve at low H 2 O 2 concentrations (<10 μM). Agarose‐coated electrode chips are used for the measurement of H 2 O 2 in the gaseous phase. Detection of H 2 O 2 is shown in a static and in a flow‐through setup. We find a limit of detection of approximately 42 ppb. Current work focuses on expanding the presented device to detection of H 2 O 2 in exhaled breath condensate.