Quantitative microfluidic analysis of   S  ‐ and   R  ‐type endotoxin components with chip capillary electrophoresis | Zendy

Makszin Lilla | Zendy; Kilár Anikó | Zendy; Felső Péter | Zendy; Péterfi Zoltán | Zendy; Kocsis Béla | Zendy; Kilár Ferenc | Zendy

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Quantitative microfluidic analysis of S ‐ and R ‐type endotoxin components with chip capillary electrophoresis

Author(s) -

Makszin Lilla,

Kilár Anikó,

Felső Péter,

Péterfi Zoltán,

Kocsis Béla,

Kilár Ferenc

Publication year - 2012

Publication title -

electrophoresis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.666

H-Index - 158

eISSN - 1522-2683

pISSN - 0173-0835

DOI - 10.1002/elps.201200167

Subject(s) - chemistry , chromatography , capillary electrophoresis , electrophoresis , bacteria , covalent bond , fluorescence , staining , silver stain , microbiology and biotechnology , biology , genetics , physics , organic chemistry , quantum mechanics

A novel, fast, and sensitive ME method was developed to analyze and differentiate the smooth ( S ) and rough ( R ) type bacterial endotoxin components labeled covalently with a fluorescent dye. The quantitative analysis of purified lipopolysaccharides, or partially purified samples from whole‐cell lysates becomes possible with this method. Two groups with three sub‐groups in the first group of S ‐type lipopolysaccharides can be classified based on the electrophoretic profiles. The LOD of the endotoxins from S ‐ and R ‐type G ram‐negative bacteria was found to be 2.6 ng and 6.9 ng, respectively. This method is capable to replace the commonly used SDS ‐ PAGE combined with silver staining.

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