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Quantitative microfluidic analysis of S ‐ and R ‐type endotoxin components with chip capillary electrophoresis
Author(s) -
Makszin Lilla,
Kilár Anikó,
Felső Péter,
Péterfi Zoltán,
Kocsis Béla,
Kilár Ferenc
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200167
Subject(s) - chemistry , chromatography , capillary electrophoresis , electrophoresis , bacteria , covalent bond , fluorescence , staining , silver stain , microbiology and biotechnology , biology , genetics , physics , organic chemistry , quantum mechanics
A novel, fast, and sensitive ME method was developed to analyze and differentiate the smooth ( S ) and rough ( R ) type bacterial endotoxin components labeled covalently with a fluorescent dye. The quantitative analysis of purified lipopolysaccharides, or partially purified samples from whole‐cell lysates becomes possible with this method. Two groups with three sub‐groups in the first group of S ‐type lipopolysaccharides can be classified based on the electrophoretic profiles. The LOD of the endotoxins from S ‐ and R ‐type G ram‐negative bacteria was found to be 2.6 ng and 6.9 ng, respectively. This method is capable to replace the commonly used SDS ‐ PAGE combined with silver staining.