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K + , N a + , and M g 2+ on DNA translocation in silicon nitride nanopores
Author(s) -
Uplinger James,
Thomas Brian,
Rollings Ryan,
Fologea Daniel,
McNabb David,
Li Jiali
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200165
Subject(s) - nanopore , silicon nitride , chemistry , dna , chromosomal translocation , counterion , molecule , electrophoresis , crystallography , ion , analytical chemistry (journal) , biophysics , silicon , chromatography , biochemistry , nanotechnology , materials science , biology , organic chemistry , gene
In this work, we report on how salt concentration and cation species affect DNA translocation in voltage‐biased silicon nitride nanopores. The translocation of ds DNA in linear, circular, and supercoiled forms was measured in salt solutions containing KC l, N a C l, and M g C l 2 . As the KC l concentrations were decreased from 1 to 0.1 M, the time taken by a DNA molecule to pass through a nanopore was shorter and the frequency of the translocation in a folded configuration was reduced, suggesting an increase in DNA electrophoretic mobility and DNA persistence length. When the salt concentration was kept at 1 M, but replacing K + with Na + , longer DNA translocation times ( t d ) were observed. The addition of low concentrations of M g C l 2 with 1.6 M KC l resulted in longer t d and an increased frequency of supercoiled DNA molecules in a branched form. These observations were consistent with the greater counterion charge screening ability of N a + and M g 2+ as compared to K + . In addition, we demonstrated that ds DNA molecules indeed translocated through a ∼10 nm nanopore by PCR amplification and gel electrophoresis. We also compared the dependence of DNA mobility and conformation on KC l concentration and cation species measured at single molecule level by silicon nitride nanopores with existing bulk‐based experimental results and theoretical predictions.