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Different strategies for the preconcentration and separation of parabens by capillary electrophoresis
Author(s) -
Maijó Irene,
Borrull Francesc,
Aguilar Carme,
Calull Marta
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200147
Subject(s) - chromatography , chemistry , capillary electrophoresis , detection limit , analytical chemistry (journal)
Several strategies, namely, large volume sample stacking ( LVSS ), field‐amplified sample injection ( FASI ), sweeping, and in‐line SPE‐CE , were investigated for the simultaneous separation and preconcentration of a group of parabens. A BGE consisting of 20 m M sodium dihydrogenphosphate (p H 2.28) and 150 m M SDS with 15% ACN was used for the separation and preconcentration of the compounds by sweeping, and a BGE consisting of 30 m M sodium borate (p H 9.5) was used for the separation and preconcentration of the compounds by LVSS , FASI , and in‐line SPE‐CE . Several factors affecting the preconcentration process were investigated in order to obtain the maximum enhancement of sensitivity. The LOD s obtained for parabens were in the range of 18–27, 3–4, 2, and 0.01–0.02 ng/m L , and the sensitivity evaluated in terms of LOD s was improved up to 29‐, 77‐, 120‐, and 18 400‐fold for sweeping, LVSS , FASI , and in‐line SPE‐CE , respectively. These preconcentration techniques showed potential as good strategies for focusing parabens. The four methods were validated with standard samples to show the potential of these techniques for future applications in real samples, such as biological and environmental samples.