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Retrotransposon‐microsatellite amplified polymorphism, an electrophoretic approach for studying genetic variability among Schistosoma japonicum geographical isolates
Author(s) -
Li Juan,
Zhao GuangHui,
Zhou DongHui,
Sugiyama Hiromu,
Nisbet Alasdair J.,
Li XiaoYan,
Zou FengCai,
Li HaiLong,
Ai Lin,
Zhu XingQuan
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200110
Subject(s) - schistosoma japonicum , biology , genetic diversity , upgma , population , polymorphism (computer science) , genetics , genetic variation , microbiology and biotechnology , genotype , gene , zoology , schistosomiasis , helminths , demography , sociology
In the present study, retrotransposon‐microsatellite amplified polymorphism ( REMAP ) was used to examine genetic variability among Schistosoma japonicum isolates from different endemic provinces in mainland C hina, using S. japonicum from J apan and the P hilippines for comparison. Of the 50 primer combinations screened, eight produced highly reproducible REMAP fragments. Using these primers, 190 distinct DNA fragments were generated in total, of which 147 (77.37%) were polymorphic, indicating considerable genetic variation among the 43 S. japonicum isolates examined. The percentage of polymorphic bands ( PPB ) among S. japonicum isolates from mainland C hina, J apan, and the P hilippines was 77.37%; PPB values of 18.42% and 53.68% were found among isolates from southwestern ( SW ) C hina and the lower Y angtze/ Z hejiang province in eastern (E) C hina, respectively. Based on REMAP profiles, unweighted pair‐group method with arithmetic averages (UPGMA) dendrogram analysis revealed that all of the S. japonicum samples grouped into three distinct clusters: parasites from mainland C hina, J apan, and the P hilippines were clustered in each individual clade. Within the mainland C hina cluster, SW C hina isolates (from S ichuan and Y unnan provinces) grouped together, whereas worms from E C hina ( Z hejiang, A nhui, J iangxi, J iangsu, H unan, and H ubei provinces) grouped together. These results demonstrated that the REMAP marker system provides a reliable electrophoretic technique for studying genetic diversity and population structures of S. japonicum isolates from mainland C hina, and could be applied to other pathogens of human and animal health significance.