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Electrowetting on dielectric actuation of droplets with capillary electrophoretic zones for MALDI mass spectrometric analysis
Author(s) -
Gorbatsova Jelena,
Borissova Maria,
Kaljurand Mihkel
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200096
Subject(s) - electrowetting , analytical chemistry (journal) , chemistry , capillary action , capillary electrophoresis , chromatography , microfluidics , conductivity , detection limit , thermal conductivity detector , desorption , analyte , digital microfluidics , detector , dielectric , mass spectrometry , materials science , electrode , nanotechnology , optoelectronics , gas chromatography , adsorption , composite material , organic chemistry , engineering , electrical engineering
An automated fraction collection interface was developed for coupling CE with MALDI‐MS. This fraction collection approach is based on the electrowetting on dielectric ( EWOD ) phenomenon performed on a digital microfluidic ( DMF ) board; it does not rely on a MALDI spotter. In this study, a four‐peptide mixture was used as a sample test, and the separations were conducted in a portable CE instrument with a 150 μm o.d. × 50 μm i.d. capillary and a contactless conductivity detector. The CE instrument was interfaced with a robust DMF board. The CE fractions were directly deposited onto the DMF board at predetermined locations prior to MALDI analysis. The series of experiments determined the lowest concentration that produces a measurable MALDI signal. The concentrations were 0.25, 0.5, 0.05, and 0.05 nmol for bradykinin, angiotensin, ACTH (18–39), and insulin, respectively. The contactless conductivity detector limit of detection for the same analytes was 2.5 μmol.