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Simultaneous determination of monoamines in rat brain with P t/ MWCNT s@ P dop hybrid nanocomposite using capillary electrophoresis— amperometric detection
Author(s) -
Liu Xiaoqian,
Yu Yanyan,
Gu Hui,
Zhou Tianshu,
Wang Lili,
Mei Bing,
Shi Guoyue
Publication year - 2013
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200071
Subject(s) - monoamine neurotransmitter , capillary electrophoresis , amperometry , nanocomposite , materials science , electrode , analytical chemistry (journal) , chromatography , chemistry , nanotechnology , electrochemistry , serotonin , biochemistry , receptor
In this article, polydopamine ( P dop) functionalized multiwalled carbon nanotubes ( MWCNT s@ P dop) were firstly synthesized and employed as the template for the dispersion of P t nanoparticles to form P t/ MWCNT s@ P dop hybrid nanocomposite. The characterizations of the P t/ MWCNT s@ P dop were investigated by SEM , transmission electron microscopy, x‐ray diffraction, and voltammetry. Based upon the P t/ MWCNT s@ P dop nanocomposite, a modified carbon disk electrode was fabricated and successfully applied to the simultaneous detection of four important monoamines, including dopamine, epinephrine, norepinephrine, and serotonin, using capillary electrophoresis—amperometric detection system. Compared with the bare electrode, the four analytes at the P t/ MWCNT s@ P dop electrode exhibited higher sensitivity, broad linear responses from 0.5 to 1.0 × 10 2 nmol/L. The detection limits ( S / N = 3) were lower than 9.2 × 10 −10 mol/L. More importantly, the effect of noise exposure on monoamines ( MA s) in hippocampus and hypothalamus was investigated for the first time. Compared with the control group, the levels of the MA s all varied to different extents after rats receiving the same noise stimulation. The results showed that the proposed method could be applied to the determination of low levels of MA s in biological samples and screening the target molecules for the diagnosis and treatment of various related diseases.