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Desktop near‐field thermal‐lens microscope for thermo‐optical detection in microfluidics
Author(s) -
Smirnova Adelina,
Proskurnin Mikhail A.,
Mawatari Kazuma,
Kitamori Takehiko
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200065
Subject(s) - lens (geology) , microscope , microfluidics , detection limit , absorbance , materials science , schematic , optics , optical microscope , laser , sensitivity (control systems) , optoelectronics , nanotechnology , chemistry , physics , chromatography , scanning electron microscope , engineering , electronic engineering
A new compact near‐field desktop‐sized diode laser thermal‐lens microscope for analysis in microfluidics was proposed. A novel beam‐alignment and detection systems provided high signal stability and, along with reduced number of optical elements rendered the instrument portable. The detection of nonfluorescent model species (Fe(II)‐bathophenanthroline chelate) in water showed good linearity in the range of 5 × 10 −9 to 1 × 10 −4 M, and the limit of detection was 3.5 × 10 −9 M, which corresponded to 3.5 × 10 −7 absorbance units and provided a 20‐fold enhancement in sensitivity compared with existing schematic.