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Capillary electrophoresis/mass spectrometry relevant to pharmaceutical and biotechnological applications
Author(s) -
Pioch Markus,
Bunz SvenjaCatharina,
Neusüß Christian
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201200030
Subject(s) - capillary electrophoresis , mass spectrometry , context (archaeology) , chromatography , capillary electrophoresis–mass spectrometry , chemistry , biochemical engineering , computer science , nanotechnology , materials science , engineering , biology , electrospray ionization , paleontology
Advanced analytical techniques play a crucial role in the pharmaceutical and biotechnological field. In this context, capillary electrophoresis/mass spectrometry ( CE / MS ) has attracted attention due to efficient and selective separation in combination with powerful detection allowing identification and detailed characterization. Method developments and applications of CE / MS have been focused on questions not easily accessible by liquid chromatography/mass spectrometry ( LC / MS ) as the analysis of intact proteins, carbohydrates, and various small molecules, including peptides. Here, recent approaches and applications of CE / MS relevant to (bio)pharmaceuticals are reviewed and discussed to show actual developments and future prospects. Based on other reviews on related subjects covering large parts of previous works, the paper is focused on general ideas and contributions of the last 2 years; for the analysis of glycans, the period is extended back to 2006.