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Diamino moiety functionalized silica nanoparticles as pseudostationary phase in capillary electrochromatography separation of plant auxins
Author(s) -
Li Hui,
Ding GuoSheng,
Yue Chun-Yue,
Tang AnNa
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100710
Subject(s) - capillary electrochromatography , chemistry , moiety , auxin , nanoparticle , chromatography , acetic acid , high performance liquid chromatography , nuclear chemistry , analytical chemistry (journal) , capillary electrophoresis , organic chemistry , materials science , nanotechnology , biochemistry , gene
A novel and simple method for the preparation of silica nanoparticles having surface‐functionalized diamino moiety (d ASNP s) was reported in our paper and characterized using scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectrometry, and thermogravimetry techniques. To test this method practically, in this contribution we describe the enhanced separation of four plant auxins – indole‐3‐acetic acid ( IAA ), indole‐3‐butyric acid (IBA), 2,4‐dichlorophenoxyacetic acid (d CPAA ), and 2‐(1‐naphthyl) acetic acid ( NAA ) – by capillary electrochromatography using diamino moiety functionalized silica nanoparticles as pseudostationary phase (PSP) in the running buffer. The effect of p H , buffer concentration, and diamino moiety functionalized silica nanoparticles concentration on the selectivity of separation was investigated. A combination of the nanoparticles and running buffer reversed the electroosmotic direction making possible the rapid and efficient separation of the auxins from the auxins migrated in the same direction with the EOF under optimum experimental conditions. A good resolution of four auxins was obtained within 5.5 min under optimum experimental conditions. The precision ( RSD , n = 5) was in the range of 0.72–0.91% and 1.89–2.23% for migration time and peak area response, respectively. The detection limits were 0.48, 0.44, 0.46, and 0.42 μM for NAA , IBA , IAA , and d CPAA , respectively. Furthermore, the method was successfully tested for the determination of IAA in the grapes.