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Determination of metolcarb in food by capillary electrophoresis immunoassay with a laser‐induced fluorescence detector
Author(s) -
Liu Cuicui,
Fang Guozhen,
Deng Qiliang,
Zhang Yan,
Feng Jingjing,
Wang Shuo
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100704
Subject(s) - capillary electrophoresis , laser induced fluorescence , chromatography , immunoassay , detector , fluorescence , chemistry , analytical chemistry (journal) , optics , biology , physics , antibody , immunology
A capillary electrophoresis immunoassay ( CEIA ) was developed for the determination of trace metolcarb ( MTMC ) in food. The method was based on the competitive reactions between fluorescently labeled MTMC tracer and free MTMC with a limited amount of anti‐ MTMC antibody and the separation and determination by CE with LIF detector. A fluorescent reagent, FITC was labeled on MTMC to construct an immunofluorescent probe. CEIA experimental parameters such as the pH value and concentration of the running buffer and separation voltage as well as incubation time were systematically investigated. Under the optimized conditions, fluorescently labeled antigen and antibody bound could be well separated within 3 min using Na 2 B 4 O 7 /NaH 2 PO 4 buffer (20:10 mmol/L, pH 9.0) for background electrolyte, 20 kV for the separation voltage, and 20°C for the column temperature. The linear range of the method was 0.25–50.0 μg/L with LOD 0.07 μg/L. The RSD for relative migration time and relative fluorescence intensity ratio were 2.90% (intraday) and 4.73% (intraday), respectively. The proposed method has been applied to determine the residue of MTMC in food samples with the satisfactory recovery.