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Luminescent iridium(III) complexes as novel protein staining agents
Author(s) -
Jia Junli,
Fei Hao,
Zhou Ming
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100693
Subject(s) - iridium , staining , ruthenium , luminescence , chemistry , protein detection , tris , salt (chemistry) , ligand (biochemistry) , electrophoresis , nuclear chemistry , chromatography , biochemistry , nanotechnology , materials science , biology , organic chemistry , receptor , genetics , optoelectronics , catalysis
This article reports a new class of luminescent metal complexes, biscyclometalated iridium( III ) complexes with an ancillary bathophenanthroline disulfonate ligand, for staining protein bands that are separated by electrophoresis. The performances of these novel staining agents have been studied in comparison with tris(bathophenanthroline disulfonate) ruthenium( II ) tetrasodium salt (i.e. R u BPS ) using a commercially available imaging system. The staining agents showed different limits of detection, linear dynamic ranges, and protein‐to‐protein variations. The overall performances of all three stains were found to be better than or equivalent to R u BPS under the experimental conditions.