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Amylose‐3,5‐dimethylphenylcarbamate immobilized on monolithic silica stationary phases for chiral separations in capillary electrochromatography
Author(s) -
Liu Yueqi,
Heyden Yvan Vander,
Mangelings Debby
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100689
Subject(s) - capillary electrochromatography , monolith , monolithic hplc column , chemistry , enantiomer , chromatography , amylose , electrochromatography , capillary action , capillary electrophoresis , high performance liquid chromatography , organic chemistry , materials science , catalysis , starch , composite material
Chiral monolithic silica capillary columns were prepared by immobilization of amylose‐3,5‐dimethylphenylcarbamate ( ADMPC ) bearing a small fraction of 3‐(triethoxysilyl)propyl residues through intermolecular polycondensation of the triethoxysilyl groups. The obtained columns were used for chiral separations in capillary electrochromatography ( CEC ). The effects of the silica monolith nature and the used chiral selector concentration on the resulting enantiomeric separations were investigated. Fifteen chiral compounds, including acidic, neutral, and basic substances were evaluated and twelve showed partial or baseline separation at some of the different conditions tested. These results demonstrated the promising applicability of ADMPC ‐immobilized monolithic silica columns in CEC enantioseparations, but also revealed the need for further improvements on the level of baseline separations and efficiencies.