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Development and validation of a capillary electrophoresis method with capacitively coupled contactless conductivity detection ( CE ‐ C 4 D ) for the analysis of amikacin and its related substances
Author(s) -
ElAttug Mohamed Nouri,
Adams Erwin,
Van Schepdael Ann
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100688
Subject(s) - amikacin , capillary electrophoresis , chemistry , chromatography , ammonium acetate , analytical chemistry (journal) , kanamycin , conductivity , electrophoresis , capillary action , materials science , antibiotics , high performance liquid chromatography , biochemistry , composite material
Amikacin is a semisynthetic aminoglycoside antibiotic derived from kanamycin A that lacks a strong UV absorbing chromophore or fluorophore. Due to the physicochemical properties of amikacin and its related substances, CE in combination with capacitively coupled contactless conductivity detection ( CE ‐ C 4 D ) was chosen. The optimized separation method uses a BGE composed of 20 mM MES adjusted to pH 6.6 by l ‐histidine and 0.3 mM CTAB that was added as flow modifier in a concentration below the CMC. Ammonium acetate 20 mg.L −1 was used as internal standard. 30 kV was applied in reverse polarity on a fused silica capillary (73/48 cm; 75 μm id). The optimized separation was obtained in less than 6 min with good linearity ( R 2 = 0.9996) for amikacin base. It shows a good precision expressed as RSD on relative peak areas equal to 0.1 and 0.7% for intraday and interday, respectively. The LOD and LOQ are 0.5 mg.L −1 and 1.7 mg.L −1 , respectively.