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Quaternized celluloses as new dynamic coatings in capillary electrophoresis for basic protein separation
Author(s) -
Zhao Lingguo,
Zhou Jinping,
Xie Huan,
Huang Dihui,
Zhou Ping
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100678
Subject(s) - chymotrypsinogen , capillary electrophoresis , chromatography , chemistry , lysozyme , electrophoresis , cationic polymerization , aqueous solution , capillary action , trypsin , adsorption , chemical engineering , materials science , chymotrypsin , polymer chemistry , organic chemistry , biochemistry , composite material , enzyme , engineering
In this work, a series of quaternized celluloses ( QC s), homogeneously synthesized in the NaOH/urea aqueous solutions, were studied as dynamic coatings for capillary electrophoresis. Capillaries coated with these cationic cellulose derivatives at the concentration as low as 3 μg/mL were able to generate a stable, reversed electroosmotic flow. The effects of QC molecular parameters, such as the degree of cationic substitution and molecular weight, and the effect of buffer pH on the EOF mobility as well as the separation of basic proteins were investigated in detail. It was shown that the use of QC coatings in CE could drastically reduce the analysis time and improve the separation performance within a broad pH range. Five basic proteins, that is, lysozyme, ribonuclease A, cytochrome C, bovine pancreatic trypsin inhibitor, and chymotrypsinogen were baselinely separated even at pH 8.0. The separation efficiency and analysis reproducibility demonstrated that the QC coatings were efficient in minimizing the adsorption of basic proteins on the fused silica capillary. The successful performance was further demonstrated for biosample analysis.