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1 H‐NMR‐based metabolomic analysis of the effect of moderate wine consumption on subjects with cardiovascular risk factors
Author(s) -
VázquezFresno Rosa,
Llorach Rafael,
Alcaro Francesca,
Rodríguez Miguel Ángel,
Vinaixa Maria,
ChivaBlanch Gemma,
Estruch Ramon,
Correig Xavier,
AndrésLacueva Cristina
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100646
Subject(s) - wine , metabolome , metabolomics , metabolite , food science , urine , biology , medicine , biochemistry , bioinformatics
Moderate wine consumption is associated with health‐promoting activities. An H‐NMR‐based metabolomic approach was used to identify urinary metabolomic differences of moderate wine intake in the setting of a prospective, randomized, crossover, and controlled trial. Sixty‐one male volunteers with high cardiovascular risk factors followed three dietary interventions (28 days): dealcoholized red wine (RWD) (272mL/day, polyphenol control), alcoholized red wine (RWA) (272mL/day) and gin (GIN) (100mL/day, alcohol control). After each period, 24‐h urine samples were collected and analyzed by 1 H‐NMR. According to the results of a one‐way ANOVA, significant markers were grouped in four categories: alcohol‐related markers (ethanol); gin‐related markers; wine‐related markers; and gut microbiota markers (hippurate and 4‐hydroxphenylacetic acid). Wine metabolites were classified into two groups; first, metabolites of food metabolome: tartrate (RWA and RWD), ethanol, and mannitol (RWA); and second, biomarkers that relates to endogenous modifications after wine consumption, comprising branched‐chain amino acid (BCAA) metabolite (3‐methyl‐oxovalerate). Additionally, a possible interaction between alcohol and gut‐related biomarkers has been identified. To our knowledge, this is the first time that this approach has been applied in a nutritional intervention with red wine. The results show the capacity of this approach to obtain a comprehensive metabolome picture including food metabolome and endogenous biomarkers of moderate wine intake.