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Using electrophoretic exclusion to manipulate small molecules and particles on a microdevice
Author(s) -
Kenyon Stacy M.,
Weiss Noah G.,
Hayes Mark A.
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100622
Subject(s) - electrophoresis , microscale chemistry , rhodamine b , polystyrene , rhodamine , electrokinetic phenomena , fluorescence , microsphere , nanotechnology , chemistry , materials science , chromatography , analytical chemistry (journal) , chemical engineering , polymer , optics , physics , composite material , organic chemistry , mathematics education , mathematics , photocatalysis , engineering , catalysis
Electrophoretic exclusion, a novel separations technique that differentiates species in bulk solution using the opposing forces of electrophoretic velocity and hydrodynamic flow, has been adapted to a microscale device. Proof‐of‐principle experiments indicate that the device was able to exclude small particles (1 μm polystyrene microspheres) and fluorescent dye molecules (rhodamine 123) from the entrance of a channel. Additionally, differentiation of the rhodamine 123 and polystyrene spheres was demonstrated. The current studies focus on the direct observation of the electrophoretic exclusion behavior on a microchip.