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Development of a generic micellar electrokinetic chromatography method for the separation of 15 antimalarial drugs as a tool to detect medicine counterfeiting
Author(s) -
Lamalle Caroline,
Marini Roland Djang'Eing'A,
Debrus Benjamin,
Lebrun Pierre,
Crommen Jacques,
Hubert Philippe,
Servais AnneCatherine,
Fillet Marianne
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100621
Subject(s) - piperaquine , halofantrine , amodiaquine , proguanil , chromatography , artemether , mefloquine , artesunate , pyrimethamine , primaquine , chemistry , micellar electrokinetic chromatography , dihydroartemisinin , chloroquine , analyte , cannabinol , central composite design , quality by design , capillary electrophoresis , artemisinin , malaria , response surface methodology , plasmodium falciparum , medicine , cannabinoid , receptor , biochemistry , immunology , particle size
Since antimalarial drugs counterfeiting is dramatically present on the A frican market, the development of simple analytical methods for their quality control is of great importance. This work consists in the CE analysis of 15 antimalarials (artesunate, artemether, amodiaquine, chloroquine, piperaquine, primaquine, quinine, cinchonine, mefloquine, halofantrine, sulfadoxine, sulfalen, atovaquone, proguanil, and pyrimethamine). Since all these molecules cannot be ionized at the same pH, MEKC was preferred because it also allows separation of neutral compounds. Preliminary experiments were first carried out to select the most crucial factors affecting the antimalarials separation. Several conditions were tested and four parameters as well as their investigation domain were chosen: pH (5–10), SDS concentration (20–90 mM), ACN proportion (10–40%), and temperature (20–35°C). Then, the experimental design methodology was used and a central composite design was selected. Mathematical modeling of the migration times allowed the prediction of optimal conditions (29°C, pH 6.6, 29 mM SDS , 36% ACN ) regarding analyte separation. The prediction at this optimum was verified experimentally and led to the separation of 13 compounds within 8 min. Finally, the method was successfully applied to the quality control of A frican antimalarial medicines for their qualitative and quantitative content.