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Dietary genistein enhances phosphorylation of regulatory myosin light chain in the myocardium of ovariectomized mice
Author(s) -
Schwab Karima,
Stein Robert,
Scheler Christian,
Theuring Franz
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100607
Subject(s) - genistein , myosin light chain kinase , phosphorylation , ovariectomized rat , myosin , isoflavones , myosin light chain phosphatase , chemistry , microbiology and biotechnology , medicine , endocrinology , biology , biochemistry , estrogen
There is evidence that isoflavones, such as genistein, can directly or indirectly improve lipid profile and lower blood pressure and hence exert cardiovascular protection. It is further believed, that genistein attenuates vascular contraction and thus vascular tone and blood pressure through altering the phosphorylation of the regulatory myosin light chain ( MLC ) probably via the myosin light chain kinase ( MLCK ) or the R ho A pathway. However, the direct role of genistein in the myocardium is poorly reviewed. In this study, we investigated the impact of genistein on the cardiac proteome in ovariectomized female mice using a 2DE‐MS approach. Dietary genistein intake considerably changed the abundance of several cytoskeletal and contractile proteins and enhanced the phosphorylation of MLC . The MLC phosphorylation was mediated through increased abundance of MLCK and inhibition of myosin light chain phosphatase latest known to be inversely regulated by R ho A . Contrary to others, in our model genistein did neither inhibit the cardiac MLCK , nor the cardiac R ho A pathway in vivo.