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Chip‐based nanoflow high performance liquid chromatography coupled to mass spectrometry for profiling of soybean flavonoids
Author(s) -
Chang Yuwei,
Zhao Chunxia,
Wu Zeming,
Zhou Jia,
Zhao Sumin,
Lu Xin,
Xu Guowang
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100581
Subject(s) - chromatography , isoflavones , high performance liquid chromatography , chemistry , flavonoid , mass spectrometry , sample preparation , cultivar , biology , botany , biochemistry , antioxidant
In this work a chip‐based nano HPLC coupled MS ( HPLC ‐chip/ MS ) method with a simple sample preparation procedure was developed for the flavonoid profiling of soybean. The analytical properties of the method including the linearity ( R 2 , 0.992–0.995), reproducibility ( RSD , 1.50–7.66%), intraday precision ( RSD , 1.41–5.14%) and interday precision ( RSD , 2.76–16.90%) were satisfactory. Compared with the conventional HPLC / MS method, a fast extraction and analysis procedure was applied and more flavonoids were detected in a single run. Additionally, 13 flavonoids in soybean seed were identified for the first time. The method was then applied to the profiling of six varieties of soybean sowed at the same place. A clear discrimination was observed among different cultivars, three isoflavones, accounting for nearly 80% of total flavonoid contents, were found increased in the spring soybeans compared with the summer cultivars.