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Simultaneous determination of components released from dental composite resins in human saliva by liquid chromatography/multiple‐stage ion trap mass spectrometry
Author(s) -
Hsu WeiYi,
Wang VenShing,
Lai ChienChen,
Tsai FuuJen
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100571
Subject(s) - chromatography , chemistry , dental composite , saliva , triethylene glycol , monomer , biodegradation , mass spectrometry , derivatization , composite number , materials science , organic chemistry , polymer , composite material , biochemistry
Dental composite resins are widely used for fixing teeth; however, the monomers used in dental composite resins have been found to be cytotoxic and genotoxic, namely triethylene glycol dimethacrylate (TEGDMA), urethane dimethacrylate (UDMA), and bisphenol A glycol dimethacrylate (Bis‐GMA). In this study, we incubated dental composite resins with human saliva for demonstrating the released monomers and biodegradation products. A simple saliva sample dilution method without purification or derivatization was used for quantification. We found that liquid chromatography coupled with multiple‐stage ion trap mass spectrometry (LC‐MS n ) operated in selected reaction monitoring (SRM) mode was able to separate the three monomers within 10 min. The calibration curves were linear ( R 2 >0.996) over a wide range for each monomer in saliva: TEGDMA, 5–500 ppb; UDMA, 5–100 ppb, and Bis‐GMA, 5–700 ppb. Furthermore, several biodegradation products were discovered with data‐dependent MS/MS scan techniques. Although TEGMA degradation products have previously been reported, we identified two previously unknown UDMA degradation products. The LC‐MS/MS method developed in this study was able to successfully quantify monomers and their principal biodegradation products from dental composite resins in human saliva.