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Development of multiplex PCR system with 15 X ‐ STR loci and genetic analysis in three nationality populations from C hina
Author(s) -
Liu QiuLing,
Lu DeJian,
Quan Li,
Chen YeFei,
Shen Min,
Zhao Hu
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100558
Subject(s) - biology , genetics , multiplex , locus (genetics) , microbiology and biotechnology , allele , multiplex polymerase chain reaction , population , allele frequency , microsatellite , polymerase chain reaction , gene , demography , sociology
The aim of this study is to develop a new multiplex PCR system that simultaneously amplifies the 15 X ‐chromosome short tandem repeats ( X ‐ STR s) loci in the same PCR reaction, and to obtain the 15 X ‐ STR loci database in three nationality populations from C hina. This multiplex system includes DXS 7133, DXS 6801, DXS 981, DXS 6809, DXS 7424, DXS 6789, DXS 9898, DXS 7132, GATA 165 B 12, DXS 101, DXS 10075, DXS 6800, GATA 31 E 08, DXS 10074, and DXS 10079, which were successfully analyzed on 1251 DNA samples (670 males and 581 females) from G uangdong H an population, X injiang U igur and K azakh. The allele frequencies and mutation rates of the 15 loci were investigated, and the allele frequency distribution among different populations was compared. A total of 6–17 alleles for each locus were observed and altogether 170 alleles for all the selected loci were found. Thirteen cases with mutation of the above loci were detected in 11 850 meioses. Pairwise comparisons of the allele frequencies distribution showed significant differences in most loci among different populations. The results indicate that this multiplex system may provide high polymorphism information for kinship testing and relationship investigations, and it is necessary to gain allele frequency and mutation rate of different population for forensic application.