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E xploring of tri‐allelic SNP s using P yrosequencing and the SN a P shot methods for forensic application
Author(s) -
Zha Lagabaiyila,
Yun Libing,
Chen Pengyu,
Luo Haibo,
Yan Jing,
Hou Yiping
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100508
Subject(s) - snp , single nucleotide polymorphism , multiplex , allele , genetics , typing , biology , snp genotyping , genotype , polymerase chain reaction , gene
T ri‐allelic single nucleotide polymorphisms ( SNP s) are potential forensic markers for DNA analysis. Currently, only a limited number of tri‐allelic SNP loci have been proved to be fit for forensic application. In this study, we aimed to develop an effective method to select and genotype tri‐allelic SNPs based on both Pyrosequencing ( PSQ ) and the SNaPshot methods. 50 candidate SNP s were chosen from NCBI 's db SNP database and were analyzed by PSQ . The results revealed that 20 SNP s were tri‐allelic and were located on 16 autosomal chromosomes. Then 20 SNP loci were combined in one multiplex polymerase chain reaction to develop a single base extension ( SBE )‐based SNP ‐typing assay. A total of 100 unrelated Chinese individuals were genotyped by this assay and allele frequencies were estimated. The total discrimination power was 0.75 and the cumulative probability of exclusion was 0.9937. These data demonstrated that the strategy is a rapid and effective method for seeking and typing tri‐allelic SNP s. In addition, the 20 tri‐allelic SNP multiplex typing assay may be used to supplement paternity testing and human identification.