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High‐voltage power supplies to capillary and microchip electrophoresis
Author(s) -
Blanes Lucas,
Coltro Wendell Karlos Tomazelli,
Saito Renata Mayumi,
Van Gramberg Amanda,
Lucio do Lago Claudimir,
Doble Philip
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100490
Subject(s) - capillary electrophoresis , electrokinetic phenomena , high voltage , instrumentation (computer programming) , electrophoresis , chromatography , voltage , materials science , process (computing) , analytical chemistry (journal) , computer science , chemistry , nanotechnology , electrical engineering , engineering , operating system
Over the past years, the development of capillary electrophoresis ( CE ) and microchip electrophoresis ( ME ) systems has grown due to instrumental simplicity and wide application. In both CE and ME , the application of a high voltage ( HV ) is a crucial step in the electrokinetic ( EK ) injection and separation processes. Particularly on ME devices, EK injection is often performed with three different modes: gated, pinched, and unpinched. In all these cases, different potential values may be applied to one or multiple channels to control the injection of small sample volumes as well as the separation process. For this reason, the construction of reliable HV power supplies ( HVPS ) is required. This review covers the advances of the development of commercial and laboratory‐built HVPS for CE and ME . Moreover, it intends to be a guide for new developers of electrophoresis instrumentation.