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Analysis of iron‐phytosiderophore complexes in soil related samples: LC‐ESI‐MS/MS versus CE‐MS
Author(s) -
Dell'mour Madeleine,
Schenkeveld Walter,
Oburger Eva,
Fischer Lisa,
Kraemer Stephan,
Puschenreiter Markus,
Lämmerhofer Michael,
Koellensperger Gunda,
Hann Stephan
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100466
Subject(s) - chemistry , ammonium acetate , chromatography , extraction (chemistry) , diastereomer , detection limit , siderophore , analytical chemistry (journal) , high performance liquid chromatography , stereochemistry , biochemistry , gene
Phytosiderophores (PS) form stable complexes with various transition metals. These ligands are exuded by the roots of graminacous plants as a mechanism for mobilizing and acquiring soil iron. To investigate iron mobilization and transport, a novel LC method in combination with ESI‐MS/MS for the determination of three Fe(III)‐complexes with mugineic acid (MA), 2′‐epi‐MA and 2′‐deoxymugineic acid (DMA) has been developed. Liquid chromatographic separation was realized using a silica‐based mixed‐mode reversed phase/weak‐anion exchange type stationary phase and a 50 mM ammonium acetate buffer, pH 6.5. Baseline separation of the two complex diastereomers Fe(III)‐MA and Fe(III)‐ epi ‐MA could be achieved. ESI‐MS/MS detection allowed for simultaneous quantification of the complexes and the free ligands. Limits of detection were determined to be 0.001 and 0.05 μM for DMA and Fe(III)‐DMA, respectively. The analytical figures of merit of the novel method were evaluated and compared with a CE‐ESI‐MS method that we had published earlier. The LC‐ESI‐MS/MS method has been successfully applied to real samples derived from preliminary extraction experiments.