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Trivalent copper chelate‐luminol chemiluminescence system for highly sensitive CE detection of dopamine in biological sample after clean‐up using SPE
Author(s) -
Wang Lin,
Liu Ying,
Xie Haoyue,
Fu Zhifeng
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100423
Subject(s) - chemiluminescence , luminol , copper , chelation , chemistry , dopamine , chromatography , environmental chemistry , inorganic chemistry , organic chemistry , biology , neuroscience
A transition metal chelate unstable at a high oxidation state, diperiodatocuprate (III) ( K 5 [ C u( HIO 6 ) 2 ], DPC ), was synthesized and applied in the luminol‐based chemiluminescence ( CL ) system for highly sensitive CE end‐column detection of dopamine ( DA ). This method was based on the fact that DA enhanced the CL emission resulting from the reaction between luminol and DPC in alkaline medium. The DPC ‐luminol‐ DA CL system showed very intensive emission and very fast kinetic characteristics, thus resulting in a high sensitivity in flow‐through detection mode for CE . Under optimal conditions, the linear range was 1.0 × 10 −8 –5.0 × 10 −5 g/mL ( R 2 = 0.9984) with a limit of detection of 6.0 × 10 −9 g/mL ( S / N = 3). The RSDs of the peak height and the migration time were about 4.2 and 2.4% for a standard sample at 3.0 × 10 −6 g/mL ( n = 5), respectively. The presented method has been successfully used for the determination of DA in commercial preparation and human urine samples after clean‐up using SPE .