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The occurrence of genetic polymorphism and related non‐allelic proteins increases the compositional complexity of goat α (s1) ‐ CN
Author(s) -
Garro Giuseppina,
Ferranti Pasquale,
De Pascale Sabrina,
Nicolai Maria Adalgisa,
Mauriello Rosalba,
Quarto Maria,
Pilla Fabio,
Chianese Lina
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100408
Subject(s) - allele , exon , genetics , biology , polymorphism (computer science) , casein , phenotype , genetic variants , gene , genotype , biochemistry
A genetic survey on three autochthonous goat breeds reared in Italy was carried out by a proteomic approach. This methodology, further to providing the phenotypic frequency of identified α s1 genetic variants, allowed to determine (i) the additional constitutive presence of a non‐allelic ‘α s1 ‐ casein (CN) F like’ protein in goat ‘strong’ α s1 variants; (ii) an α s1 ‐ CN B 2 like protein, expressed at very low quantitative level, in goat ‘weak’ α s1 ‐ CN variants, and, as main focus; (iii) the occurrence of a new α s1 ‐ CN D 1 variant characterised by the lack of α s1 (f59‐69) sequence otherwise encoded by exon 9 in goat α s1 B 2 reference. The same exon skipping event had been identified since 1990, as responsible of the ‘weak quantitative class’ of α s1 ‐ CN D variant (0.6 g/L), while the new α s1 ‐ CN D 1, has been ‘quantitatively’ classified as an ‘intermediate’ variant, since 1.8 g/L per allele was assessed in the milk.