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Analysis of drugs of forensic interest with capillary zone electrophoresis/time‐of‐flight mass spectrometry based on the use of non‐volatile buffers
Author(s) -
Gottardo Rossella,
Mikšík Ivan,
Aturki Zeineb,
Sorio Daniela,
Seri Catia,
Fanali Salvatore,
Tagliaro Franco
Publication year - 2012
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100383
Subject(s) - chromatography , capillary electrophoresis , chemistry , mass spectrometry , capillary electrophoresis–mass spectrometry , time of flight mass spectrometry , electrospray ionization , analyte , ammonium formate , analytical chemistry (journal) , ionization , ion , organic chemistry
he present work is aimed at investigating the influence of the background electrolyte composition and concentration on the separation efficiency and resolution and mass spectrometric detection of illicit drugs in a capillary zone electrophoresis‐electrospray ionization‐time of flight mass spectrometry (CZE‐ESI‐TOF MS) system. The effect of phosphate, borate and Tris buffers on the separation and mass spectrometry response of a mixture of 3,4‐methylenedioxyamphetamine, 3,4‐methylenedioxymethamphetamine, methadone, cocaine, morphine, codeine and 6‐monoacetylmorphine was studied, in comparison with a reference ammonium formate separation buffer. Inorganic non‐volatile borate and Tris buffers proved hardly suitable for capillary electrophoresis‐mass spectrometry (CE‐MS) analysis, but quite unexpectedly ammonium phosphate buffers showed good separation and ionization performances for all the analytes tested. Applications of this method to real samples of hair from drug addicts are also provided.