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Rapid detection of dysfunctional high‐density lipoproteins using isoelectric focusing‐based microfluidic device to diagnose senescence‐related disease
Author(s) -
Jang Wookju,
Shim Jaesool,
Lee DongYeon,
Dutta Prashanta,
Kim JaeRyong,
Cho KyungHyun
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100361
Subject(s) - isoelectric point , isoelectric focusing , chemistry , medicine , chromatography , biochemistry , enzyme
Recently, we reported elevated levels of advanced glycated end products (AGEs) in human high‐density lipoproteins (HDL), with fragmentation of apoA‐I in an elderly group, compared with a younger group. More dysfunctional HDL from human plasma was demonstrated in the elderly group, including reconstituted HDL containing glycated apoA‐I (gA‐I‐rHDL) with elevation of AGEs. Based on SDS‐PAGE analysis, HDL 3 from the elderly group (E‐HDL 3 ) exhibited increased multimerization with increased smear band intensity compared to HDL 3 from the younger group (Y‐HDL 3 ). According to isoelectric focusing gel analysis, gA‐I‐rHDL and E‐HDL 3 showed electromobility to the basic region of pH with a broader band range. In a microfluidic channel, E‐HDL 3 had faster mobility with a broader range and a higher isoelectric point (p I , approximately 8.1), whereas Y‐HDL 3 showed a narrow band range with a lower p I (approximately 6.9). In conclusion, gA‐I‐rHDL and E‐HDL share several electrophoretic properties with multimerization and faster mobility in microfluidic channels, depending on the isoelectric point. These results can be applied to develop a rapid detection system for modified HDL to predict the extent of aging and aging‐related metabolic diseases, such as cardiovascular disease and diabetes.