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A prototypic system of parallel electrophoresis in multiple capillaries coupled with microwell arrays
Author(s) -
Su Jing,
Ren Kangning,
Dai Wen,
Zhao Yihua,
Zhou Jianhua,
Wu Hongkai
Publication year - 2011
Publication title -
electrophoresis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.666
H-Index - 158
eISSN - 1522-2683
pISSN - 0173-0835
DOI - 10.1002/elps.201100339
Subject(s) - microfluidics , polydimethylsiloxane , interfacing , electrode array , capillary electrophoresis , electrophoresis , capillary action , materials science , nanotechnology , chemistry , electrode , chromatography , computer science , computer hardware , composite material
We present a microfluidic system that can be directly coupled with microwell array and perform parallel electrophoresis in multiple capillaries simultaneously. The system is based on an array of glass capillaries, fixed in a polydimethylsiloxane (PDMS) microfluidic scaffold, with one end open for interfacing with microwells. In this capillary array, every two adjacent capillaries act as a pair to be coupled with one microwell; samples in the microwells are introduced and separated by simply applying voltage between two electrodes that are placed at the other ends of capillaries; thus no complicated circuit design is required. We evaluate the performance of this system and perform multiple CE with direct sample introduction from microwell array. Also with this system, we demonstrate the analysis of cellular contents of cells lysed in a microwell array. Our results show that this prototypic system is a promising platform for high‐throughput analysis of samples in microwell arrays.